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KMID : 0382619820020010027
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Hanyang Journal of Medicine
1982 Volume.2 No. 1 p.27 ~ p.37
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Pyocine type and their reproducibility of Pseudomonas aeruginosa by Gillies-Govan´s method
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金基豪/Kim, Ki Ho
韓王洙/徐仁銖/Han, Wang Soo/Suh, Inn Soo
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Abstract
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A total of two hundred and eighty-nine strains isolated during 1978 to 1980 in Seoul:area were typed according to their ability to produce pyocines by the method of Gillies and Govan using ox blood trypticase soy agar. The strains of Pseudomonas were isolated from pus, sputum, urine, feces, blood, nasal and throat discharges, wound, hospital environment and unknown origin. The typing was repeated three, times at an interval of about one month and serogically typing according to Homma et al. using same cultures were carried out. Three and two out of three results of pyocin typing came to a concurrence: 49. 1 % , and 45. 3% respectively in the original table and 5.5 % non-reproducible (NR) strains.
Two out of three results came to a concurrence: 98 strains of Type 1, 44 strains of Type 3, 30 strains of Type 10, 25 strains of non-producer strains (NT), 15 strains of Type 6, 6 strains each of Type 5 and 35, 5 strains of Type 22, 3 strains each of Type 2 and 23, 2 strains each of Type 18, 27, 29, 30, 31, and 33, 1 straineach of Type 11 and 26 in, the original table and- 24.UC strains. Eighteen pyocintypes were distributed.
There was a difference in distribution of type by the year of isolation and specimen source and pyocine typing was reliable method to monitor the patterns of infections due to Pseudomonas aeruginosa.
The correlation between pyocine type and serotype were found namely in 1 andB, I and F, and non-producer (NT) and I.
On the other hand, the method of pyocine and serotyping were useful for sub typing one another.
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